From promoter motif to cardiac function: A single DPE motif affects transcription regulation and organ function in vivo.
Sloutskin, A.*, ‡, Itzhak, D.*, Vogler, G., Pozeilov, H., Ideses, D., Alter, H., Adato, O., Shachar, H., Doniger, D., Shohat-Ophir, G., Frasch, M., Bodmer, R., Duttke, S.H. and Juven-Gershon, T. (2024) Development, dev.202355. PubMed

*The first two authors contributed equally to this paper
co-corresponding authors


ElemeNT 2023: an enhanced tool for detection and curation of core promoter elements
Adato, O.*, Sloutskin, A.*, Komemi, H., Brabb, I., Duttke, S., Bucher, P., Unger, R. ‡, and Juven-Gershon, T. ‡ (2024) Bioinformatics, btae110. online publication   PubMed

*The first two authors contributed equally to this paper
co-corresponding authors


CAGI, the Critical Assessment of Genome Interpretation, establishes progress and prospects for computational genetic variant interpretation methods
Critical Assessment of Genome Interpretation Consortium, (2024) Genome Biology, 25(1):53. online publication  



Rapid Biosensing Method for Detecting Protein–DNA Interactions
Roth, S., Ideses, D., Juven-Gershon, T. and Danielli, A. (2022) ACS Sens. online publication    PubMed



Changing and stable chromatin accessibility supports transcriptional overhaul during neural stem cell activation and is altered with age

Maybury-Lewis, S.Y., Brown, A.K., Yeary, M., Sloutskin, A., Dhakal, S, Juven-Gershon, T. and Webb, A.E. (2021) Aging Cell, e13499           PubMed

The core promoter is a regulatory hub for developmental gene expression

Sloutskin, A., Shir-Shapira, H., Freiman, R. and Juven-Gershon, T. (2021) Frontiers in Cell and Developmental Biology, 9:666508         PubMed   Full Text


Computational identification and experimental characterization of preferred downstream positions in human core promoters

Dreos, R.*, Sloutskin, A.*, Malachi, N.*, Ideses, D., Bucher, P. and Juven-Gershon, T. (2021) PLOS Computational Biology, 17(8):e1009256          PubMed  Full Text

*The first two authors contributed equally to this paper
co-corresponding authors



Quantitative analysis of differential expression of HOX genes in multiple cancers

Adato, O., Orenstein, Y., Kopolovic, J., Juven-Gershon, T. and Unger, R. (2020) Cancers (Basel). 12(6):1572       PubMed   Full Text

co-corresponding authors


Efficient in vivo introduction of point mutations using ssODN and a Co-CRISPR approach

Levi, T.*, Sloutskin, A.*, Kalifa, R., Juven-Gershon, T. and Gerlitz, O. (2020) Biol Proced Online. 22:14  PubMed   Full Text

co-corresponding authors



Integration of multiple epigenomic marks improves prediction of variant impact in saturation mutagenesis reporter assay

Shigaki, D., Adato, O., Adhikari, AN., Dong, S.,Hawkins-Hooker, A., Inoue, F., Juven-Gershon, T., Kenlay, H., Martin, B., Patra, A., Penzar, DD., Schubach, M., Xiong, C., Yan, Z., Boyle, AP., Kreimer, A., Kulakovskiy, IV., Reid, J., Unger, R., Yosef, N., Shendure, J., Ahituv, N., Kircher, M., Beer, MA. (2019) Hum Mutat  40(9): 1280-1291       PubMed


Identification of evolutionarily conserved downstream core promoter elements required for the transcriptional regulation of Fushi tarazu target genes

Shir-Shapira, H.*, Sloutskin, A.*, Adato, O., Ovadia-Shochat, A., Ideses, D., Zehavi, Y., Kassavetis, G., Kadonaga, J.T., Unger, R. and Juven-Gershon, T. (2019) PLoS ONE. 14(4): e0215695   PubMed   Full Text



Functional Screening of Core Promoter Activity

Even, D.Y., Kedmi, A., Ideses, D. and Juven-Gershon, T. (2017) Methods Mol Biol. 1651, 77-91     PubMed


Structural and dynamics characterization of the MerR family metalloregulator CueR in its repression and activation states

Sameach, H., Narunsky, A., Azoulay-Ginsburg, S., Gevorkyan-Aiapetov, L., Zehavi, Y., Moskovitz, Y., Juven-Gershon, T., Ben-Tal, N. and Ruthstein, S. (2017)  Structure. 25(7), 988-996.e3       PubMed



SELMAP – SELEX affinity landscape MAPping of transcription factor binding sites using integrated microfluidics

Chen, D., Orenstein Y., Golodnitsky R., Pellach M., Avrahami D., Wachtel C., Ovadia-Shochat A., Shir-Shapira H., Kedmi A., Juven-Gershon T., Shamir R., and Gerber D. (2016) Scientific Reports, 6, 33351       PubMed


Engineered Promoters for Potent Transient Overexpression

Even, D.Y.*, Kedmi A.*, Basch-Barzilay, S.*, Ideses, D., Tikotzki, R., Shir-Shapira, H., Shefi, O. and Juven-Gershon, T. (2016)  PLOS ONE, 11(2): e0148918     PubMed   Full Text



ElemeNT: A Computational Tool for Detecting Core Promoter Elements

Sloutskin, A., Danino, YM., Orenstein, Y., Zehavi, Y., Doniger, T., Shamir, R. and Juven-Gershon, T. (2015)  Transcription, 6(3), 41-50      PubMed   Full Text

Here you can find the described resources.


Structure-Function Analysis of the Drosophila melanogaster Caudal provides insights into core promoter-preferential activation

Shir-Shapira, H.*, Sharabany,J.*, Filderman, M., Ideses, D., Ovadia-Shochat, A., Mannervik, M. and Juven-Gershon, T. (2015)  Journal of Biological Chemistry, 290(28) 17293-305     PubMed   Full Text


The core promoter: at the heart of gene expression

Danino,Y.M, Even, D., Ideses, D.,  and Juven-Gershon,T. (2015)  BBA Gene Regulatory Mechanisms, 1849(8), 1116-31       PubMed   Full Text


TRF2: TRansForming the view of general transcription factors

Zehavi, Y.*, Kedmi, A.*, Ideses, D., and Juven-Gershon, T. (2015) Transcription, 6(1), 1-6  PubMed   Full Text



The FOXO Transcription Factor DAF-16 Bypasses ire-1 Requirement to Promote Endoplasmic Reticulum Homeostasis

Safra M., Fickentscher R., Levi-Ferber M., Danino Y.M., Haviv-Chesner A., Hansen M., Juven-Gershon T., Weiss M., Henis-Korenblit S. (2014)  Cell Metabolism, 20, 870-881   PubMed 


Drosophila TRF2 Is a Preferential Core Promoter Regulator

Kedmi, A.*,  Zehavi, Y*.,  Glick, Y., Orenstein, Y., Ideses, D., Wachtel, C.,  Doniger, T., Waldman Ben-Asher, H., Munster, N., Thompson, J., Anderson, S., Avrahami, D., Yates, JR 3rd, Shamir, R., Gerber, D., and Juven-Gershon, T.  (2014) Genes & Development, 28, 2163-2174    PubMed   Full Text


The core promoter composition establishes a new dimension in developmental gene networks

Zehavi, Y., Sloutskin, A., Kuznetsov, O., and Juven-Gershon, T. (2014)  Nucleus, 5:4,298–303 PubMed   Full Text


Core promoter functions in the regulation of gene expression of Drosophila Dorsal target genes

Zehavi, Y., Kuznetsov, O., Ovadia-Shochat, A. and Juven-Gershon, T. (2014)  Journal of Biological Chemistry, 289, 11993-12004          PubMed   Full Text



Human TFIID binds to core promoter DNA in a reorganized structural state

Cianfrocco, M.A., Kassavetis, G.A., Grob, P., Fang, J., Juven-Gershon, T., Kadonaga, J.T. and Nogales, E. (2013)  Cell, 152, 120-131       PubMed   Full Text



Regulation of gene expression via the core promoter and the basal transcriptional machinery

Juven-Gershon, T. and Kadonaga, J.T. (2010) Developmental Biology, 339, 225-229        PubMed   Full Text

-One of the top-five most cited articles published in the journal Developmental
Biology during the period 1/1/2009-31/12/2011 



Caudal, a key developmental regulator, is a DPE-specific transcriptional factor

Juven-Gershon, T., Hsu, J.-Y. and Kadonaga, J.T. (2008) Genes & Development, 22, 2823-2830       PubMed   Full Text


TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription

Hsu, J.-Y, Juven-Gershon, T., Marr, M.T. 2nd, Wright, K.J., Tjian, R. and Kadonaga, J.T. (2008) Genes & Development, 22, 2353-2358       PubMed   Full Text


The RNA polymerase II core promoter – the gateway to transcription

Juven-Gershon, T., Hsu, J.-Y. Theisen J.W.M. and Kadonaga, J.T. (2008) Current Opinion in Cell Biology, 20, 253-259    PubMed   Full Text



Perspectives on the RNA polymerase II core promoter

Juven-Gershon, T., Hsu, J.-Y. and Kadonaga, J.T. (2006) Biochemical Society Transactions 34, 1051-1054         PubMed   Full Text


Rational design of a super core promoter that enhances gene expression

Juven-Gershon, T., Cheng, S. and Kadonaga, J.T. (2006) Nature Methods, 3, 917 –922     PubMed

-Potential applications of this study were discussed in: Perkel, J.M. (2007)
Studies you can use. The Scientist, 21,63


2001 and earlier

Siah-1 binds and regulates the function of Numb

Susini, L.*,Passer, B.J.*,Amzallag-Elbaz, N.*, Juven-Gershon, T.*, Prieur, S., Privat, N., Tuynder, M, Gendron M., Israel, A., Amson, R., Oren, M. and Telerman, A. (2001)  Proc. Natl. Acad. Sci. USA, 98, 15067-15072  PubMed   Full Text

*The first four authors contributed equally to this paper


Critical role for Ser20 of human p53 in the negative regulation of p53 by Mdm2

Unger, T.*, Juven-Gershon, T.*, Moallem, E.*, Berger, M., Vogt-Sionov, R., Lozano, G., Oren, M. and Haupt, Y. (1999)  EMBO J., 18, 1805-1814          PubMed   Full Text

*The first three authors contributed equally to this paper


The c-fos proto-oncogene is a target for transactivation by the p53 tumor suppressor

Elkeles, A., Juven-Gershon, T., Israeli, D., Wilder, S., Zalcenstein, A. and Oren, M. (1999)  Molecular & Cellular Biology, 19, 2594-2600         PubMed   Full Text


Mdm2: the ups and the downs

Juven-Gershon, T. and Oren, M. (1999)  Molecular Medicine, 5, 71-83          PubMed   Full Text


The Mdm2 oncoprotein interacts with the cell fate regulator Numb

Juven-Gershon, T., Shifman, O., Unger, T., Elkeles, A., Haupt, Y. and Oren, M. (1998)  Molecular & Cellular Biology, 18, 3974-3982    PubMed   Full Text


Regulation of mdm2 expression by p53: alternative promoters produce transcripts with nonidentical translation potential

Barak, Y., Gottlieb, E., Juven-Gershon, T. and Oren, M. (1994) Genes & Development, 8, 1739-1749                   PubMed   Full Text


Targets for transcriptional activation by wild-type p53: endogenous retroviral LTR, immunoglobulin-like promoter, and an internal promoter of the mdm2 gene

Barak, Y., Lupo, A., Zauberman, A., Juven, T., Aloni-Grinstein, R., Gottlieb, E., Rotter, V. and Oren, M. (1994)  Cold Spring Harb Symp. Quant. Biol., 59, 225-235               PubMed


Antibodies to different isoforms of the heavy neurofilament protein (NF- H) in normal aging and Alzheimer’s disease

Soussan, L., Tchernakov, K., Bachar-Lavi, O., Juven, T., Wertman, E. and Michaelson, D.M. (1994) Molecular Neurobiology, 9, 83-91    PubMed


Wild type p53 can mediate sequence-specific transactivation of an internal promoter within the mdm2 gene

Juven, T., Barak, Y., Zauberman, A., George, D.L. and Oren, M. (1993) Oncogene, 8, 3411-3416    PubMed


mdm2 expression is induced by wild type p53 activity

Barak, Y., Juven, T., Haffner, R. and Oren, M. (1993) EMBO J., 12, 461-468          PubMed   Full Text


Optimized core promoters and uses thereof

Kadonaga and Gershon. Patent no. US 7,968,698 B2: 2006.